4O2B
Tubulin-Colchicine complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-03 |
| Detector | PSI PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.310, 157.010, 180.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.700 - 2.300 |
| R-factor | 0.183 |
| Rwork | 0.182 |
| R-free | 0.21300 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 4i4t |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.651 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.700 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.115 | 1.943 |
| Number of reflections | 149841 | |
| <I/σ(I)> | 21.3 | 1.7 |
| Completeness [%] | 99.7 | 97.9 |
| Redundancy | 13.6 | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 | 293 | 6% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 100 mM MES/Imidazole , pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
