4O1O
Crystal Structure of RNase L in complex with 2-5A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97925 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.792, 111.968, 268.465 |
Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
Resolution | 49.700 - 3.270 |
R-factor | 0.23225 |
Rwork | 0.230 |
R-free | 0.28851 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o1p |
RMSD bond length | 0.010 |
RMSD bond angle | 1.394 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.700 | 3.352 |
High resolution limit [Å] | 3.250 | 3.267 |
Number of reflections | 51209 | |
Completeness [%] | 94.1 | 92.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 5 mM DTT, 18% PEG2000, 100 mM NaCl, and 100 mM SPG buffer, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |