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4O1O

Crystal Structure of RNase L in complex with 2-5A

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 24-ID-C
Synchrotron siteAPS
Beamline24-ID-C
Temperature [K]100
Detector technologyPIXEL
Collection date2013-11-13
DetectorDECTRIS PILATUS 6M
Wavelength(s)0.97925
Spacegroup nameP 1 21 1
Unit cell lengths59.792, 111.968, 268.465
Unit cell angles90.00, 90.03, 90.00
Refinement procedure
Resolution49.700 - 3.270
R-factor0.23225
Rwork0.230
R-free0.28851
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4o1p
RMSD bond length0.010
RMSD bond angle1.394
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwareREFMAC (5.7.0032)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]49.7003.352
High resolution limit [Å]3.2503.267
Number of reflections51209
Completeness [%]94.192.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP72935 mM DTT, 18% PEG2000, 100 mM NaCl, and 100 mM SPG buffer, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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