4NZ7
Steroid receptor RNA Activator (SRA) modification by the human Pseudouridine Synthase 1 (hPus1p): RNA binding, activity, and atomic model
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 273 |
Detector technology | CCD |
Collection date | 2012-11-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.8726 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 39.446, 69.044, 116.797 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.879 - 2.700 |
R-factor | 0.1889 |
Rwork | 0.183 |
R-free | 0.23810 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.863 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.770 |
High resolution limit [Å] | 2.700 | 12.080 | 2.700 |
Number of reflections | 9281 | ||
Completeness [%] | 99.8 | 96.3 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 17% PEG 8000, 0.1M HEPES, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |