4NZ0
The EMCV 3Dpol structure at 2.8A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-02 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 230.400, 140.786, 170.579 |
| Unit cell angles | 90.00, 125.86, 90.00 |
Refinement procedure
| Resolution | 138.200 - 2.800 |
| R-factor | 0.247 |
| Rwork | 0.246 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u09 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.768 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 138.200 | 2.840 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.620 | |
| Number of reflections | 119540 | |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 98.0 | 92.5 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 293 | 4.0M Ammonium acetate, 0.1M Sodium acetate pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
