4NY0

Crystal structure of FERM domain of human focal adhesion kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.115869
Spacegroup name	P 1 21 1
Unit cell lengths	71.960, 152.500, 95.340
Unit cell angles	90.00, 93.62, 90.00

Refinement procedure

Resolution	95.150 - 2.800
R-factor	0.1952
Rwork	0.193
R-free	0.23020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.409
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	95.150	95.150	2.930
High resolution limit [Å]	2.800	5.600	2.800
Rmerge	0.047	0.018	0.651
Number of reflections	49012
<I/σ(I)>		22.2	1.4
Completeness [%]		99.9	79.5
Redundancy	2	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293.15	17% PEG 10000, 0.1M Ammonium Sulfate, 0.1M bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K