4NWU
Crystal structure of APE1551, an anti-human NGF Fab with a nine amino acid insertion in CDR H1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2013-06-23 |
| Detector | PILATUS 6M-PAD |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.053, 66.256, 63.884 |
| Unit cell angles | 90.00, 108.54, 90.00 |
Refinement procedure
| Resolution | 45.580 - 1.602 |
| R-factor | 0.16787 |
| Rwork | 0.166 |
| R-free | 0.19767 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | APE1531 Fab |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.984 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.580 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.067 | 0.623 |
| Number of reflections | 64694 | |
| <I/σ(I)> | 23.9 | 2.4 |
| Completeness [%] | 97.9 | 96.3 |
| Redundancy | 6.6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 16% PEG 6000, 0.1 M Citric acid pH6.0-7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
