4NWT
Crystal structure of the anti-human NGF Fab APE1531
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2012-12-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97830 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 127.720, 64.360, 63.810 |
Unit cell angles | 90.00, 108.67, 90.00 |
Refinement procedure
Resolution | 38.640 - 1.750 |
R-factor | 0.15194 |
Rwork | 0.150 |
R-free | 0.18121 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3QOT for VHVL 3SOB for CH1CL |
RMSD bond length | 0.019 |
RMSD bond angle | 1.988 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.640 | 1.800 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.048 | 0.604 |
Number of reflections | 48714 | |
<I/σ(I)> | 25.3 | 2.9 |
Completeness [%] | 98.5 | 97.7 |
Redundancy | 6.9 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 12% PEG 20000, 0.1M NaCl, 0.1M MES pH6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |