4NUG
Crystal structure of HIV-1 broadly neutralizing antibody PGT151
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-11 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 118.645, 66.977, 84.552 |
Unit cell angles | 90.00, 133.91, 90.00 |
Refinement procedure
Resolution | 33.489 - 1.862 |
R-factor | 0.1613 |
Rwork | 0.159 |
R-free | 0.20660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fx7 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.095 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.500 | 1.890 |
High resolution limit [Å] | 1.860 | 1.860 |
Number of reflections | 39140 | |
<I/σ(I)> | 29.2 | 4.6 |
Completeness [%] | 97.2 | |
Redundancy | 3.4 | 74.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.2 | 293.15 | 40% PEG 3350, 0.1M phosphate-citrate, pH 4.2, VAPOR DIFFUSION, temperature 293.15K |