4NUB
Crystal structure of Escherichia coli ribosomal oxygenase YcfD
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-09 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97913, 0.97927, 0.91837 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 75.731, 75.731, 210.898 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.926 - 2.700 |
R-factor | 0.1806 |
Rwork | 0.178 |
R-free | 0.22830 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.218 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.700 |
Rmerge | 0.070 |
Number of reflections | 31993 |
<I/σ(I)> | 11.5 |
Completeness [%] | 99.9 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 3.6% PEG 4000, 30% Glycerol, 0.07M sodium acetate, 0.5 uL of 1/10 dilution of seed solution, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |