4NTW
Structure of acid-sensing ion channel in complex with snake toxin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | H 3 |
Unit cell lengths | 151.930, 151.930, 123.790 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.000 - 2.070 |
R-factor | 0.2083 |
Rwork | 0.206 |
R-free | 0.24580 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.089 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1402)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | 2.040 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.354 | |
Number of reflections | 70881 | |
<I/σ(I)> | 1.6 | |
Completeness [%] | 93.8 | 57.7 |
Redundancy | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 50 mM sodium acetate, 22-25% PEG 400, 10 mM magnesium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |