4NSQ
Crystal structure of PCAF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97916 |
Spacegroup name | P 43 |
Unit cell lengths | 65.570, 65.570, 187.622 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.256 - 2.311 |
R-factor | 0.2288 |
Rwork | 0.229 |
R-free | 0.27510 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.105 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 | 2.350 |
High resolution limit [Å] | 2.310 | 2.490 | 2.310 |
Number of reflections | 30459 | ||
Completeness [%] | 99.3 | 97.6 | 57.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.4 | 298 | 0.2M Lithium Sulfate, 0.1M HEPES pH7.4, 1.2M Ammonium Sulfate and 10mM Trimethylamine HCl, VAPOR DIFFUSION, temperature 298K |