4NRU
Murine Norovirus RNA-dependent-RNA-polymerase in complex with Compound 6, a suramin derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-04 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.220, 162.420, 122.960 |
| Unit cell angles | 90.00, 97.04, 90.00 |
Refinement procedure
| Resolution | 61.090 - 2.300 |
| R-factor | 0.194 |
| Rwork | 0.191 |
| R-free | 0.25396 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.333 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.960 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 188102 | |
| Completeness [%] | 99.9 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.4 | 293.15 | 1.6M (NH4)2SO4, 12% Glycerol, 100mM TrisHCL pH8.4, microbatch, temperature 293.15K |
