4NPL
Crystal structure of Zebrafish ALKBH5 in complex with alpha-ketoglutarate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 180 |
Detector technology | CCD |
Collection date | 2013-08-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9676 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.948, 68.595, 114.909 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.827 - 1.651 |
R-factor | 0.2098 |
Rwork | 0.209 |
R-free | 0.23790 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.140 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.650 |
Number of reflections | 60317 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.2 M Sodium iodide, pH 7.0, 20% w/v Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |