4NOD
Distinct structural features of TFAM drive mitochondrial DNA packaging versus transcriptional activation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.372, 82.490, 104.222 |
| Unit cell angles | 79.86, 85.48, 84.53 |
Refinement procedure
| Resolution | 35.640 - 2.897 |
| R-factor | 0.22345 |
| Rwork | 0.221 |
| R-free | 0.26744 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tq6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.391 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | |
| High resolution limit [Å] | 2.897 | 2.897 |
| Rmerge | 0.038 | |
| Number of reflections | 35526 | |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 50.0 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 12% PEG8000, 22% glycerol, 0.04 M potassium phosphate monobasic, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
