4NOD
Distinct structural features of TFAM drive mitochondrial DNA packaging versus transcriptional activation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 |
Unit cell lengths | 56.372, 82.490, 104.222 |
Unit cell angles | 79.86, 85.48, 84.53 |
Refinement procedure
Resolution | 35.640 - 2.897 |
R-factor | 0.22345 |
Rwork | 0.221 |
R-free | 0.26744 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tq6 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.391 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.000 | |
High resolution limit [Å] | 2.897 | 2.897 |
Rmerge | 0.038 | |
Number of reflections | 35526 | |
<I/σ(I)> | 13.5 | |
Completeness [%] | 50.0 | |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 12% PEG8000, 22% glycerol, 0.04 M potassium phosphate monobasic, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |