4NO7
Human Glucokinase in complex with a nanomolar activator.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-11-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0001 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.300, 81.300, 85.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.740 - 1.700 |
| R-factor | 0.19125 |
| Rwork | 0.190 |
| R-free | 0.22260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v4s |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.787 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.740 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.770 | |
| Number of reflections | 47815 | |
| <I/σ(I)> | 1.5 | |
| Completeness [%] | 100.0 | 88.6 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 30% PEG 4000, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
