4NO7
Human Glucokinase in complex with a nanomolar activator.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2008-11-21 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0001 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.300, 81.300, 85.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.740 - 1.700 |
R-factor | 0.19125 |
Rwork | 0.190 |
R-free | 0.22260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v4s |
RMSD bond length | 0.011 |
RMSD bond angle | 1.787 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.740 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.770 | |
Number of reflections | 47815 | |
<I/σ(I)> | 1.5 | |
Completeness [%] | 100.0 | 88.6 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 30% PEG 4000, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |