4NM5
Crystal structure of GSK-3/Axin complex bound to phosphorylated Wnt receptor LRP6 c-motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 81.727, 81.727, 280.937 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.237 - 2.300 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.23190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nm7 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.631 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.26) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.240 | 2.380 | |
| High resolution limit [Å] | 2.300 | 2.300 | |
| Total number of observations | 44079 | 7655 | |
| Number of reflections | 25685 | ||
| <I/σ(I)> | 18.9 | 1 | |
| Completeness [%] | 99.7 | 99.6 | |
| Redundancy | 18.9 | 18.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.5 | 277 | 10% PEG 35,000, 20mM Tris 7.5, 300mM NaCl, 5% glycerol, 10mM MgCl2, 200uM ATP, and 5mM DTT, MICRODIALYSIS, temperature 277K |
