4NLW

Poliovirus Polymerase - G289A/C290I Loop Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-10
Detector	NOIR-1
Spacegroup name	P 65
Unit cell lengths	128.845, 128.845, 111.733
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.222
Rwork	0.222
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ra6
Data reduction software	d*TREK (9.4L)
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.071	0.361
Number of reflections	54829
<I/σ(I)>	13.9	4.9
Completeness [%]	99.8	100
Redundancy	7.43	7.23

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	289	Grown in sodium acetate, cacodylate, DTT. Transferred to 250 mM sodium acetate, 30% (w/v) PEG-400, 0.1 M cacodylic acid and 2 mM DTT, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K