4NLS
Poliovirus Polymerase - S288A Loop Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-06 |
Detector | NOIR-1 |
Wavelength(s) | 1.000 |
Spacegroup name | P 65 |
Unit cell lengths | 129.304, 129.304, 112.512 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
Rwork | 0.234 |
R-free | 0.25640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ra6 |
Data reduction software | d*TREK (9.4LDz) |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.320 | 42.320 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.063 | 0.030 | 0.431 |
Total number of observations | 49984 | 48397 | |
Number of reflections | 87774 | ||
<I/σ(I)> | 10.7 | 30.9 | 2.4 |
Completeness [%] | 100.0 | 99.9 | 99.9 |
Redundancy | 6.78 | 6.81 | 6.68 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | Grown in sodium acetate, cacodylate, DTT. Transferred to 250 mM sodium acetate, 30% (w/v) PEG-400, 0.1 M cacodylic acid and 2 mM DTT, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K |