4NLC
Crystal structure of the catalytic core of RRP6 from Trypanosoma brucei, mutant C496S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.410, 93.410, 49.990 |
| Unit cell angles | 90.00, 105.23, 90.00 |
Refinement procedure
| Resolution | 48.230 - 2.150 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.22080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nlb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 19025 | |
| <I/σ(I)> | 9.1 | 1.6 |
| Completeness [%] | 99.5 | 98.5 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 25% PEG2000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
