4NHA

Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 16-mer siRNA having 5'-p and UU-3' ends (3.4 Angstrom resolution)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97947
Spacegroup name	I 41 2 2
Unit cell lengths	174.469, 174.469, 74.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.752 - 3.401
R-factor	0.2808
Rwork	0.277
R-free	0.31740
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.191
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.520
High resolution limit [Å]	3.400	3.400
Rmerge	0.069	0.382
Number of reflections	8091
<I/σ(I)>	30.4	4.28
Completeness [%]	99.4
Redundancy	6.8	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2 M potassium chloride, 0.1 M magnesium acetate, 0.05 M sodium cacodylate, pH 6.5, 12 % PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K