4NHA
Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 16-mer siRNA having 5'-p and UU-3' ends (3.4 Angstrom resolution)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97947 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 174.469, 174.469, 74.757 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.752 - 3.401 |
R-factor | 0.2808 |
Rwork | 0.277 |
R-free | 0.31740 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.191 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.520 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.069 | 0.382 |
Number of reflections | 8091 | |
<I/σ(I)> | 30.4 | 4.28 |
Completeness [%] | 99.4 | |
Redundancy | 6.8 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M potassium chloride, 0.1 M magnesium acetate, 0.05 M sodium cacodylate, pH 6.5, 12 % PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |