4NEF
X-ray structure of human Aquaporin 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-29 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 42 |
| Unit cell lengths | 119.110, 119.110, 90.621 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.960 - 2.750 |
| R-factor | 0.203 |
| Rwork | 0.202 |
| R-free | 0.22510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.130 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 119.110 | 3.000 |
| High resolution limit [Å] | 2.750 | 2.850 |
| Rmerge | 0.735 | |
| Number of reflections | 33049 | |
| <I/σ(I)> | 9.9 | 1 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 4.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 277 | 22% PEG400, 0.1M Tris, 0.1M NaCl, 0.15M MgCl2. 0.1M CdCl2, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
