4NEF
X-ray structure of human Aquaporin 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-29 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 42 |
Unit cell lengths | 119.110, 119.110, 90.621 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.960 - 2.750 |
R-factor | 0.203 |
Rwork | 0.202 |
R-free | 0.22510 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.130 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 119.110 | 3.000 |
High resolution limit [Å] | 2.750 | 2.850 |
Rmerge | 0.735 | |
Number of reflections | 33049 | |
<I/σ(I)> | 9.9 | 1 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 4.2 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 277 | 22% PEG400, 0.1M Tris, 0.1M NaCl, 0.15M MgCl2. 0.1M CdCl2, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |