4NCL
Crystal structure of eukaryotic translation initiation factor eIF5B (517-970) from Chaetomium thermophilum in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-04-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.826580 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.900, 72.960, 199.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.865 - 2.115 |
R-factor | 0.2157 |
Rwork | 0.214 |
R-free | 0.24780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 4N3N |
RMSD bond length | 0.005 |
RMSD bond angle | 0.956 |
Data scaling software | XSCALE |
Phasing software | PHASER (2.3.0) |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 2.210 | ||
High resolution limit [Å] | 2.120 | 50.000 | 2.120 |
Rmerge | 0.056 | 0.631 | |
Number of reflections | 56555 | ||
<I/σ(I)> | 17.79 | 2.97 | |
Completeness [%] | 99.7 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 15 % PEG 8000, 0.5 M Li2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |