4NC9
Crystal structure of phosphatidyl mannosyltransferase PimA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0668 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 137.620, 137.620, 168.641 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.520 - 3.192 |
| R-factor | 0.2141 |
| Rwork | 0.212 |
| R-free | 0.25620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gek |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.903 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.520 | 3.360 |
| High resolution limit [Å] | 3.190 | 3.190 |
| Rmerge | 0.061 | 0.512 |
| Number of reflections | 31022 | |
| Completeness [%] | 99.6 | 96.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 15% PEG 4000, 200 mM MgCl2, 100 mM Tris-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 8.0 |
