4N5T
The 1.7A Crystal Structure of MDMX with a Stapled Peptide, ATSP-7041
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.99980 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.585, 108.535, 30.964 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.070 - 1.700 |
| R-factor | 0.21 |
| Rwork | 0.209 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.600 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNX (2005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.790 | 1.550 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.046 | |
| Number of reflections | 23886 | |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 278 | 15% PEG 4000, 0.1M sodium citrate, 0.2M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
