4MVD
Crystal Structure of a Mammalian Cytidylyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-07 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.105 |
| Spacegroup name | P 43 |
| Unit cell lengths | 107.780, 107.780, 575.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 107.800 - 8.000 |
| R-factor | 0.38019 |
| Rwork | 0.379 |
| R-free | 0.40659 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.800 | 8.400 |
| High resolution limit [Å] | 8.000 | 8.000 |
| Rmerge | 0.180 | 0.660 |
| Number of reflections | 6964 | |
| <I/σ(I)> | 9.3 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1 M MES (pH 6.5), 1.8 M Ammonium Sulphate, 3 % dioxane, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
