4MSX

Crystal structure of an essential yeast splicing factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1159
Spacegroup name	P 43 21 2
Unit cell lengths	139.315, 139.315, 86.737
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.831 - 1.870
R-factor	0.1855
Rwork	0.184
R-free	0.22010
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.605
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.511	1.970
High resolution limit [Å]	1.870	1.870
Rmerge	0.085	0.945
Number of reflections	70404
<I/σ(I)>		1.7
Completeness [%]	99.8	100
Redundancy	7.7	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	13% PEG4000, 0.1 M sodium acetate, 0.1 M magnesium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	13% PEG4000, 0.1 M sodium acetate, 0.1 M magnesium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K