4MQ3
The 1.1 Angstrom Structure of Catalytic Core Domain of FIV Integrase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 103 |
| Detector technology | PIXEL |
| Collection date | 2013-07-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.254, 66.643, 32.619 |
| Unit cell angles | 90.00, 103.43, 90.00 |
Refinement procedure
| Resolution | 33.320 - 1.080 |
| R-factor | 0.14173 |
| Rwork | 0.140 |
| R-free | 0.17650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f9k |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.942 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.320 | 1.135 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Rmerge | 0.030 | |
| Number of reflections | 47812 | |
| <I/σ(I)> | 15.94 | 2.59 |
| Completeness [%] | 95.6 | 90.3 |
| Redundancy | 3.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 0.2M Trimethylamine N-oxide, 0.1M Tris pH 8.5, 20% PEG MME 2000, VAPOR DIFFUSION, temperature 293K |
