4MQ3
The 1.1 Angstrom Structure of Catalytic Core Domain of FIV Integrase
Summary for 4MQ3
| Entry DOI | 10.2210/pdb4mq3/pdb |
| Descriptor | Integrase (2 entities in total) |
| Functional Keywords | alpha beta, ribonuclease-h like motif, nucleotidyltransferase, viral protein |
| Biological source | Feline immunodeficiency virus (FIV) |
| Total number of polymer chains | 1 |
| Total formula weight | 14667.78 |
| Authors | Galilee, M.,Alian, A. (deposition date: 2013-09-15, release date: 2014-09-17, Last modification date: 2023-09-20) |
| Primary citation | Galilee, M.,Alian, A. Identification of phe187 as a crucial dimerization determinant facilitates crystallization of a monomeric retroviral integrase core domain. Structure, 22:1512-1519, 2014 Cited by PubMed Abstract: Retroviral DNA integration into the host genome is mediated by nucleoprotein assemblies containing tetramers of viral integrase (IN). Whereas the fully active form of IN comprises a dimer of dimers, the molecular basis of IN multimerization has not been fully characterized. IN has consistently been crystallized in an analogous dimeric form in all crystallographic structures and experimental evidence as to the level of similarity between IN monomeric and dimeric conformations is missing because of the lack of IN monomeric structures. Here we identify Phe187 as a critical dimerization determinant of IN from feline immunodeficiency virus (FIV), a nonprimate lentivirus that causes AIDS in the natural host, and report, in addition to a canonical dimeric structure of the FIV IN core-domain, a monomeric structure revealing the preservation of the backbone structure between the two multimeric forms and suggest a role for Phe187 in "hinging" the flexible IN dimer. PubMed: 25199694DOI: 10.1016/j.str.2014.08.001 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.08 Å) |
Structure validation
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