4MMS

Crystal Structure of Prefusion-stabilized RSV F Variant Cav1 at pH 5.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	200
Detector technology	CCD
Collection date	2013-06-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 41 21 2
Unit cell lengths	170.753, 170.753, 163.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.859 - 2.397
R-factor	0.1886
Rwork	0.187
R-free	0.21390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jhw
RMSD bond length	0.003
RMSD bond angle	0.836
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.397	2.400
Number of reflections	90864
<I/σ(I)>		2.1
Completeness [%]	95.6	97.7
Redundancy		4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	1.7 M ammonium sulfate, 0.1 M citrate pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K