4MLV
Crystal Structure of Bacillus megaterium porphobilinogen deaminase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.320, 65.780, 97.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.750 - 1.455 |
| R-factor | 0.14331 |
| Rwork | 0.141 |
| R-free | 0.18559 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pda |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.438 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.600 | 1.530 |
| High resolution limit [Å] | 1.455 | 1.455 |
| Rmerge | 0.061 | 0.559 |
| Number of reflections | 60772 | |
| <I/σ(I)> | 14.4 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M sodium cacodylate, 0.2M magnesium acetate, 25-30% PEG 8K, pH 6.5 - 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
