4MHL
The crystal structure of human interleukin-11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 118.862, 28.169, 41.806 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.810 - 2.090 |
| R-factor | 0.18855 |
| Rwork | 0.184 |
| R-free | 0.24155 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1alu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.389 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.810 | 2.150 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.090 | 0.459 |
| Number of reflections | 8862 | |
| <I/σ(I)> | 11.5 | 2.9 |
| Completeness [%] | 99.6 | 97.5 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 1.25 M ammonium sulfate, 0.2 M lithium sulfate, 0.1 M bis-tris propane, 10% formamide, 0.02% sodium azide, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
