4MHG

Crystal structure of ETV6 bound to a specific DNA sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9753
Spacegroup name	P 31 2 1
Unit cell lengths	57.582, 57.582, 130.481
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.340 - 2.199
R-factor	0.1773
Rwork	0.175
R-free	0.22140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.325
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.199	5.970	2.199
Rmerge	0.085	0.036	0.711
Number of reflections	13307
<I/σ(I)>	10.7
Completeness [%]	99.7	93.9	100
Redundancy	7.9	6.8	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	22% PEG 8000, 50 mM Sodium Cacodylate, 100 mM NH4Ac, 10 mM MgCl2, pH 6.0, vapor diffusion, hanging drop, temperature 298K