4MH5
Crystal structure of the kainate receptor GluK3 ligand binding domain in complex with (S)-glutamate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9184 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 67.800, 67.800, 122.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.897 - 1.650 |
R-factor | 0.1317 |
Rwork | 0.129 |
R-free | 0.18140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ycj |
RMSD bond length | 0.010 |
RMSD bond angle | 1.271 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.897 | 27.897 | 1.740 |
High resolution limit [Å] | 1.650 | 5.220 | 1.650 |
Rmerge | 0.033 | 0.373 | |
Number of reflections | 35179 | ||
<I/σ(I)> | 7 | 14.6 | 2 |
Completeness [%] | 100.0 | 99.1 | 100 |
Redundancy | 7.8 | 7 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | 1.8 M sodium/potassium phosphate, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |