4M8O
TERNARY COMPLEX OF DNA POLYMERASE EPSILON WITH AN INCOMING dATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.873 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 148.495, 68.919, 149.852 |
Unit cell angles | 90.00, 109.50, 90.00 |
Refinement procedure
Resolution | 47.050 - 2.200 |
R-factor | 0.183 |
Rwork | 0.180 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3iay |
RMSD bond length | 0.016 |
RMSD bond angle | 1.866 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.050 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.548 | |
Number of reflections | 69054 | |
<I/σ(I)> | 2.5 | |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 20% PEG-3350, 50 mM Hepes-NaOH pH 7.0, 10 mM MgCl2, 400 mM LiAc, and 10 mM 2-aminoethanesulfonic acid (taurine) , VAPOR DIFFUSION, HANGING DROP, temperature 291K |