4M8N
Crystal Structure of PlexinC1/Rap1B Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | .97926 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.276, 84.734, 138.752 |
| Unit cell angles | 91.09, 95.15, 90.32 |
Refinement procedure
| Resolution | 41.053 - 3.294 |
| R-factor | 0.2449 |
| Rwork | 0.243 |
| R-free | 0.29950 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.702 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.294 | 3.294 |
| Number of reflections | 47245 | |
| <I/σ(I)> | 18.6 | 1.6 |
| Completeness [%] | 91.0 | 89.2 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 294.15 | 0.1 M HEPES, 5% 2-propanol, 25% PEG 3350, 3.6% polypropylene glycol P400, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 294.15K |
