4M7D
Crystal structure of Lsm2-8 complex bound to the RNA fragment CGUUU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.539, 78.483, 142.987 |
| Unit cell angles | 90.00, 103.17, 90.00 |
Refinement procedure
| Resolution | 34.184 - 2.595 |
| R-factor | 0.242 |
| Rwork | 0.240 |
| R-free | 0.28280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m7a |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.465 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.690 |
| High resolution limit [Å] | 2.595 | 2.595 |
| Number of reflections | 41448 | |
| Completeness [%] | 98.7 | 95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 100mM Sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
