4M73

Mutant structure of methyltransferase from Streptomyces hygroscopicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A
Synchrotron site	NSRRC
Beamline	BL15A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-19
Detector	RAYONIX MX300HE
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	60.989, 96.810, 135.404
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.050 - 2.000
R-factor	0.15506
Rwork	0.152
R-free	0.21062
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kib
RMSD bond length	0.020
RMSD bond angle	2.032
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (MR)
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	48442
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	16% PEG3350, 0.2M sodium iodine, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K