4M62
Ontogeny of recognition specificity and functionality for the anti-HIV neutralizing antibody 4E10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-16 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.880, 103.480, 109.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 68.880 - 1.800 |
R-factor | 0.19076 |
Rwork | 0.189 |
R-free | 0.21608 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.699 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.880 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 71260 | |
Completeness [%] | 98.0 | 98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 0.1M Ac, pH 5.0, 1.6M Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |