4M52
Structure of Mtb Lpd bound to SL827
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.521, 111.953, 98.034 |
| Unit cell angles | 90.00, 94.43, 90.00 |
Refinement procedure
| Resolution | 39.009 - 2.400 |
| R-factor | 0.1721 |
| Rwork | 0.169 |
| R-free | 0.22180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a8x |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.880 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.097 | 0.475 |
| Number of reflections | 67889 | |
| <I/σ(I)> | 15.5 | 3.6 |
| Completeness [%] | 96.1 | 95.7 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 100 mM Tris pH 8.5, 50 mM NaCl, 19% PEG 10000 (w/v), VAPOR DIFFUSION, HANGING DROP, temperature 291K |
