4M1V
Crystal structure of the ancestral soluble variant of the Human Phosphate Binding Protein (HPBP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97655 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.040, 71.990, 38.980 |
| Unit cell angles | 90.00, 103.12, 90.00 |
Refinement procedure
| Resolution | 36.100 - 1.300 |
| R-factor | 0.10864 |
| Rwork | 0.107 |
| R-free | 0.14253 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v3q |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.381 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.910 | 1.400 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.041 | 0.275 |
| Number of reflections | 81625 | |
| <I/σ(I)> | 21.36 | 4.65 |
| Completeness [%] | 98.8 | 99.3 |
| Redundancy | 3.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | The protein was concentrated at 4mg/mL. The conditions are 0.2M sodium acetate, 0.1M sodium cacodylate, 25% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6.5 |
