4M1V

Crystal structure of the ancestral soluble variant of the Human Phosphate Binding Protein (HPBP)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Phosphate-binding protein	polymer	378	39002.3	1	UniProt (P85173) Pfam (PF12849) In PDB	unidentified prokaryotic organism	HPBP
2	A	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
3	A	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
4	A	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
5	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6		water	water		18.0	650	Chemie (HOH)

Sequence modifications

A: 1 - 376 (UniProt: P85173)

PDB	External Database	Details
Ala 0	-	expression tag
Gln 15	Leu 15	engineered mutation
Ala 37	Ile 37	engineered mutation
Ser 70	Ala 70	engineered mutation
Ser 74	Ala 74	engineered mutation
Thr 79	Gly 79	engineered mutation
Thr 102	Ala 102	engineered mutation
Thr 121	Ala 121	engineered mutation
Ser 129	Ala 129	engineered mutation
Ser 141	Ala 141	engineered mutation
Thr 162	Pro 162	engineered mutation
Gly 177	Leu 177	engineered mutation
Thr 182	Leu 182	engineered mutation
Gln 192	Asp 192	engineered mutation
Thr 196	Ala 196	engineered mutation
Met 211	Ile 211	engineered mutation
Ala 237	Val 237	engineered mutation
Val 261	Leu 261	engineered mutation
Thr 268	Gly 268	engineered mutation
Ala 283	Gly 283	engineered mutation
Thr 314	Gly 314	engineered mutation
Asn 328	Ala 328	engineered mutation
Ile 350	Val 350	engineered mutation
Leu 377	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	39002.3
Non-Polymers*	Number of molecules	9
	Total formula weight	618.5
All*	Total formula weight	39620.8

*Water molecules are not included.