4LWU
The 1.14A Crystal Structure of Humanized Xenopus MDM2 with RO5499252
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.99998 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 40.554, 78.341, 24.703 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.170 - 1.140 |
| R-factor | 0.216 |
| Rwork | 0.215 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNX (2005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.550 | 1.200 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.050 | 0.347 |
| Number of reflections | 29503 | |
| <I/σ(I)> | 15.8 | 4.6 |
| Completeness [%] | 99.1 | 95 |
| Redundancy | 5.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 278 | 40-50% Saturated Ammonium Sulfate, 0.1M MES, pH 6.5, 5% PEG200, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
