4LV8
Murine IRGa6 bound to Toxoplasma ROP5C, a pseudokinase GDI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97945 |
Spacegroup name | P 1 |
Unit cell lengths | 53.400, 53.700, 86.800 |
Unit cell angles | 104.70, 95.30, 111.00 |
Refinement procedure
Resolution | 38.669 - 1.720 |
R-factor | 0.1957 |
Rwork | 0.195 |
R-free | 0.22980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3Q60 and then 1TPZ:A using phaser |
RMSD bond length | 0.007 |
RMSD bond angle | 1.128 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.669 | 1.760 | |
High resolution limit [Å] | 1.720 | 7.690 | 1.720 |
Rmerge | 0.092 | 0.020 | 0.020 |
Number of reflections | 90541 | 998 | 6698 |
<I/σ(I)> | 19.43 | 101.58 | 1.52 |
Completeness [%] | 99.9 | 99 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 12% PEG 20,000, 0.1M MES 5.8, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |