4LTC
Crystal structure of yeast 20S proteasome in complex with enone carmaphycin analogue 6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-05 |
Detector | PSI PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 135.170, 300.230, 144.380 |
Unit cell angles | 90.00, 112.79, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.500 |
R-factor | 0.2055 |
Rwork | 0.204 |
R-free | 0.23250 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.910 |
Data scaling software | XSCALE |
Phasing software | CNS |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 2.600 | ||
High resolution limit [Å] | 2.500 | 30.000 | 2.500 |
Rmerge | 0.051 | 0.017 | 0.538 |
Number of reflections | 354256 | 112 | 39325 |
<I/σ(I)> | 16.34 | 79.32 | 1.71 |
Completeness [%] | 97.1 | 49.1 | 97.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 297 | 30 mM of magnesium acetate, 100 mM of MES (pH 7.2) and 12% of MPD, vapor diffusion, temperature 297K |