4LT5
Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-16 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 83.954, 108.648, 166.384 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.547 - 2.893 |
R-factor | 0.1944 |
Rwork | 0.193 |
R-free | 0.21540 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.675 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SGXPRO |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.990 |
High resolution limit [Å] | 2.890 | 2.890 |
Rmerge | 0.077 | 0.783 |
Number of reflections | 17328 | |
<I/σ(I)> | 22.8 | 2.1 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 6.4 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 2 M ammonium sulfate, 0.1 M BisTris-HCl, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
2 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 20% (w/v) polyethylene glycol 3350, and 0.2 M sodium citrate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |