4LOP
Structural basis of autoactivation of p38 alpha induced by TAB1 (Tetragonal crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 41 |
| Unit cell lengths | 86.540, 86.540, 226.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.550 - 2.049 |
| R-factor | 0.14995 |
| Rwork | 0.148 |
| R-free | 0.18753 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3que |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.576 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.600 | 2.160 |
| High resolution limit [Å] | 2.049 | 2.049 |
| Rmerge | 0.084 | 0.422 |
| Number of reflections | 103422 | |
| <I/σ(I)> | 8.5 | 2.1 |
| Completeness [%] | 99.5 | 97.1 |
| Redundancy | 3.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277.15 | 20% PEG 3350, 0.2 M Na/K tartrate, 0.1 M Bis-Tris propane, pH 6.5, 10% ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
