4LNX
Crystal structure of TR-alpha bound to T4 in a second site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU ULTRAX 18 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-03-19 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.744, 80.924, 101.871 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.600 - 2.050 |
R-factor | 0.157 |
Rwork | 0.155 |
R-free | 0.17700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.375 |
Data reduction software | DENZO |
Data scaling software | SCALA (3.1.9) |
Phasing software | AMoRE |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 63.365 | 101.871 | 2.160 |
High resolution limit [Å] | 2.050 | 6.480 | 2.050 |
Rmerge | 0.068 | 0.036 | 0.375 |
Number of reflections | 31309 | ||
<I/σ(I)> | 31.8 | 15 | 2 |
Completeness [%] | 98.9 | 99.8 | 97.6 |
Redundancy | 14.6 | 12.2 | 14.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | 1.0 M sodium cacodylate and 0.1 M sodium acetate threehydrate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |