4LNG
Aspergillus fumigatus protein farnesyltransferase complex with farnesyldiphosphate and tipifarnib
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.537, 90.658, 83.163 |
| Unit cell angles | 90.00, 111.09, 90.00 |
Refinement procedure
| Resolution | 30.237 - 1.905 |
| R-factor | 0.1563 |
| Rwork | 0.155 |
| R-free | 0.18450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.793 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 66308 |
| Completeness [%] | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4-10% PEG6000, 600-800 mM LiCl, 100 mM HEPES , pH 7.5, VAPOR DIFFUSION, HANGING DROP |
