4LMD
Crystal structure of the JCV large t-antigen origin binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.067, 37.133, 64.753 |
| Unit cell angles | 90.00, 112.58, 90.00 |
Refinement procedure
| Resolution | 38.064 - 1.500 |
| R-factor | 0.1685 |
| Rwork | 0.167 |
| R-free | 0.20160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.529 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.068 | 0.799 |
| Number of reflections | 51172 | |
| <I/σ(I)> | 22 | 1.5 |
| Completeness [%] | 98.7 | 90.1 |
| Redundancy | 7.4 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1 M sodium citrate, 30 % Peg 3350, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
