4LKX
Humanized antibody 4B12 Fab complexed with a CemX segment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-10-30 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97622 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.045, 107.156, 110.215 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.360 - 1.920 |
| R-factor | 0.18212 |
| Rwork | 0.180 |
| R-free | 0.22852 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jix 3aaz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.301 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.360 |
| High resolution limit [Å] | 1.920 |
| Rmerge | 0.094 |
| Number of reflections | 38689 |
| <I/σ(I)> | 29.3 |
| Completeness [%] | 99.9 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 0.1M sodium acetate trihydrate, 25% PEG 4000, 0.2M ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
